Taking Snapshots with Novel Ultrafast Vibrational Spectroscopies:Bridging the Gap between Structure and Function of Biomolecules

Biomolecules perform fascinating functions essential for all life forms, including disease states upon perturbation or mutation. In order for existing biomolecular complexes to achieve optimal performances or to engineer new ones to execute targeted activities, a deep “bottom-up” understanding of the connection between the fundamental molecular mechanism and biological functions is required. I will present my recent results on tackling this outstanding issue by utilizing two novel ultrafast vibrational spectroscopic techniques, femtosecond stimulated Raman and two-dimensional infrared, unveiling the bright fluorescence of GFP, the cross-talk between two helical strands inside membrane, and the unusual potency of a new anti-HIV/AIDS drug inhibiting its enzyme target. These unique biophysical data capture the structural snapshots of biomolecules “in action”, and offer a fresh perspective for biomedicine and bioengineering.